Keating Lab

Publications

Beyond native sequence recovery: Improved modeling of the sequence-energy landscape of protein structures
Birnbaum F, Keating AE
Proc Natl Acad Sci USA, 2026
Training Bias and Sequence Alignments Shape Protein-Peptide Docking by AlphaFold and Related Methods
Guan L, Keating AE
Protein Sci, 2025
Design of specific peptide inhibitors of toxin-antitoxin-mediated anti-phage defense
Ghose D, Swanson S, Birnbaum F, ​​​​Britton D, Gan J, Mahoney E, Laub M, Keating AE
ACS Synth Biol, 2025
Elaboration of the Homer1 recognition landscape reveals incomplete divergence of paralogous EVH1 domains
Singer A, Ramos A, Keating AE
Protein Sci, 2024
Jointly Embedding Protein Structures and Sequences through Residue Level Alignment
Birnbaum F, Jain S, Madry A, Keating AE
American Physical Society, 2024
PairK: Pairwise k-mer alignment for quantifying protein motif conservation in disordered regions
Lee T, Keating AE
Protein Sci, 2024
Peptides from human BNIP5 and PXT1 and non-native binders of pro-apoptotic BAK can directly activate or inhibit BAK-mediated membrane permeabilization
Aguilar F, Stacey Yu, Grant RA, Swanson S, Ghose D, Su BG, Sorosiek KA, Keating AE
Structure, 2023
Marginal specificity in protein interactions constrains evolution of a paralogous family
Ghose DA, Przydzial KE, Mahoney EM, Laub MT, Keating AE
Proc Natl Acad Sci USA, 2023
Tertiary motifs as building blocks for the design of protein-binding peptides
Swanson S, Sivaraman V, Grigoryan G, Keating AE
Protein Sci, 2022
Molecular determinants of TRAF6 binding specificity suggest that native interaction partners are not optimized for affinity
Halpin J, Whitney D, Rigoldi F, Sivaraman V, Singer A, Keating AE
Protein Sci, 2022
Neural network-derived Potts models for structure-based protein design using backbone atomic coordinates and tertiary motifs
Li AJ, Lu M, Desta I, Sundar V, Grigoryan G, Keating AE
Protein Sci, 2022
Data-driven computational protein design
Frappier V, Keating AE
Curr Opin Struct Biol, 2021
A distributed residue network permits conformational binding specificity in a conserved family of actin remodelers
Hwang T, Parker S, Hill SM, Ilunga MW, Grant RA, Mouneimne G, Keating AE
eLife, 2021
Native proline-rich motifs exploit sequence context to target actin-remodeling Ena/VASP proteins
Hwang T, Grant RA, Ilunga MW, Sivaraman V, Keating AE
eLife, 2021
Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator
Shemesh OA, Linghu C, Piatkevich KD, Goodwin D, Celiker OT, Gritton HJ, Romano MF, Gao R, Yu CJ, Tseng HA, Bensussen S, Narayan S, Yang CT, Freifeld L, Siciliano CA, Gupta I, Wang J, Pak N, Yoon YG, Ullmann JFP, Guner-Ataman B, Noamany H, Sheinkopf ZR, Park WM, Asano S, Keating AE, Trimmer JS, Reimer J, Tolias AS, Bear MF, Tye KM, Han X, Ahrens MB, Boyden ES
Neuron, 2020
Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics
Linghu C, Johnson SL, Valdes PA, Shemesh OA, Park WM, Park D, Piatkevich KD, Wassie AT, Liu Y, An B, Barnes SA, Celiker OT, Yao CC, Yu CJ, Wang R, Adamala KP, Bear MF, Keating AE, Boyden ES
Cell, 2020
Tertiary Structural Motif Sequence Statistics Enable Facile Prediction and Design of Peptides that Bind Anti-apoptotic Bfl-1 and Mcl-1
Frappier V, Jenson JM, Zhou J, Grigoryan G, Keating AE
Structure, 2019
PixelDB: Protein-peptide complexes annotated with structural conservation of the peptide binding mode
Frappier V, Duran M, Keating AE
Protein Sci, 2018
Iterative optimization yields Mcl-1-targeting stapled peptides with selective cytotoxicity to Mcl-1-dependent cancer cells
Rezaei Araghi R, Bird GH, Ryan JA, Jenson JM, Godes M, Pritz JR, Grant RA, Letai A, Walensky LD, Keating AE
Proc Natl Acad Sci USA, 2018
Peptide design by optimization on a data-parameterized protein interaction landscape
Jenson JM, Xue V, Stretz L, Mandal T, Reich LL, Keating AE
Proc Natl Acad Sci USA, 2018
Enriching Peptide Libraries for Binding Affinity and Specificity Through Computationally Directed Library Design
Foight GW, Chen TS, Richman D, Keating AE
Methods Mol Biol, 2017
Combinatorial bZIP dimers display complex DNA-binding specificity landscapes
Rodriguez-Martinez JA, Reinke AW, Bhimsaria D, Keating AE, Ansari AZ
eLife, 2017
Epistatic mutations in PUMA BH3 drive an alternate binding mode to potently and selectively inhibit anti-apoptotic Bfl-1
Jenson JM, Ryan JA, Grant RA, Letai A, Keating AE
eLife, 2017
Modular assembly of a protein nanotriangle using orthogonally interacting coiled coils
Park WM, Bedewy M, Berggren KK, Keating AE
Sci Rep, 2017
Generating High-Accuracy Peptide-Binding Data in High Throughput with Yeast Surface Display and SORTCERY
Reich LL, Dutta S, Keating AE
Methods Mol Biol, 2016
Selective peptide inhibitors of antiapoptotic cellular and viral Bcl-2 proteins lead to cytochrome c release during latent Kaposi's sarcoma-associated herpesvirus infection
Burrer CM, Foight GW, Keating AE, Chan GC
Virus Res, 2016
The Orientations of Large Aspect-Ratio Coiled-Coil Proteins Attached to Gold Nanostructures
Chang JB, Kim YH, Thompson E, No YH, Kim NH, Arrieta J, Manfrinato VR, Keating AE, Berggren KK
Small, 2016
Rapid Optimization of Mcl-1 Inhibitors using Stapled Peptide Libraries Including Non-Natural Side Chains
Rezaei Araghi R, Ryan JA, Letai A, Keating AE
ACS Chem Biol, 2016
Comparison of the peptide binding preferences of three closely related TRAF paralogs: TRAF2, TRAF3, and TRAF5
Foight GW, Keating AE
Protein Sci, 2016
Designing helical peptide inhibitors of protein-protein interactions
Rezaei Araghi R, Keating AE
Curr Opin Struct Biol, 2016
AB-Bind: Antibody binding mutational database for computational affinity predictions
Sirin S, Apgar JR, Bennett EM, Keating AE
Protein Sci, 2016
Selectivity in subunit composition of Ena/VASP tetramers
Riquelme DN, Meyer AS, Barzik M, Keating A, Gertler FB
Biosci Rep, 2015
Data-driven prediction and design of bZIP coiled-coil interactions
Potapov V, Kaplan JB, Keating AE
PLoS Comput Biol, 2015
Potent and specific peptide inhibitors of human pro-survival protein Bcl-xL
Dutta S, Ryan J, Chen TS, Kougentakis C, Letai A, Keating AE
J Mol Biol, 2015
SORTCERY - A High-Throughput Method to Affinity Rank Peptide Ligands
Reich LL, Dutta S, Keating AE
J Mol Biol, 2015
Locating Herpesvirus Bcl-2 Homologs in the Specificity Landscape of Anti-Apoptotic Bcl-2 Proteins
Foight GW, Keating AE
J Mol Biol, 2015
A Barcoding Strategy Enabling Higher-Throughput Library Screening by Microscopy
Chen R, Rishi HS, Potapov V, Yamada MR, Yeh VJ, Chow T, Cheung CL, Jones AT, Johnson TD, Keating AE, DeLoache WC, Dueber JE
ACS Synth Biol, 2015
Genome-wide prediction and validation of peptides that bind human prosurvival Bcl-2 proteins
DeBartolo J, Taipale M, Keating AE
PLoS Comput Biol, 2014
Increasing the affinity of selective bZIP-binding peptides through surface residue redesign
Kaplan JB, Reinke AW, Keating AE
Protein Sci, 2014
Designed BH3 peptides with high affinity and specificity for targeting Mcl-1 in cells
Foight GW, Ryan JA, Gulla SV, Letai A, Keating AE
ACS Chem Biol, 2014
A set of computationally designed orthogonal antiparallel homodimers that expands the synthetic coiled-coil toolkit
Negron C, Keating AE
J Am Chem Soc, 2014
bZIP transcription factors affecting secondary metabolism, sexual development and stress responses in Aspergillus nidulans
Yin WB, Reinke AW, Szilagyi M, Emri T, Chiang YM, Keating AE, Pocsi I, Wang CC, Keller NP
Microbiology, 2013
Multistate protein design using CLEVER and CLASSY
Negron C, Keating AE
Methods Enzymol, 2013
Structure-based redesign of the binding specificity of anti-apoptotic Bcl-x(L)
Chen TS, Palacios H, Keating AE
J Mol Biol, 2013
Helix bundle loops determine whether histidine kinases autophosphorylate in cis or in trans
Ashenberg O, Keating AE, Laub MT
J Mol Biol, 2013
Peptide ligands for pro-survival protein Bfl-1 from computationally guided library screening
Dutta S, Chen TS, Keating AE
ACS Chem Biol, 2013
Networks of bZIP protein-protein interactions diversified over a billion years of evolution
Reinke AW, Baek J, Ashenberg O, Keating AE
Science, 2013
SYNZIP protein interaction toolbox: in vitro and in vivo specifications of heterospecific coiled-coil interaction domains
Thompson KE, Bashor CJ, Lim WA, Keating AE
ACS Synth Biol, 2012
Designing specific protein-protein interactions using computation, experimental library screening, or integrated methods
Chen TS, Keating AE
Protein Sci, 2012
In silico and in vitro elucidation of BH3 binding specificity toward Bcl-2
London N, Gulla S, Keating AE, Schueler-Furman O
Biochemistry, 2012
Predictive Bcl-2 family binding models rooted in experiment or structure
DeBartolo J, Dutta S, Reich L, Keating AE
J Mol Biol, 2012
Multicoil2: predicting coiled coils and their oligomerization states from sequence in the twilight zone
Trigg J, Gutwin K, Keating AE, Berger B
PLoS One, 2011
Protein binding specificity versus promiscuity
Schreiber G, Keating AE
Curr Opin Struct Biol, 2011
Design of peptide inhibitors that bind the bZIP domain of Epstein-Barr virus protein BZLF1
Chen TS, Reinke AW, Keating AE
J Mol Biol, 2011
Determinants of homodimerization specificity in histidine kinases
Ashenberg O, Rozen-Gagnon K, Laub MT, Keating AE
J Mol Biol, 2011
Mcl-1-Bim complexes accommodate surprising point mutations via minor structural changes
Fire E, Gulla SV, Grant RA, Keating AE
Protein Sci, 2010
Identification of bZIP interaction partners of viral proteins HBZ, MEQ, BZLF1, and K-bZIP using coiled-coil arrays
Reinke AW, Grigoryan G, Keating AE
Biochemistry, 2010
Specific coiled-coil interactions contribute to a global model of the structure of the spindle pole body
Zizlsperger N, Keating AE
J Struct Biol, 2010
A synthetic coiled-coil interactome provides heterospecific modules for molecular engineering
Reinke AW, Grant RA, Keating AE
J Am Chem Soc, 2010
Determinants of BH3 binding specificity for Mcl-1 versus Bcl-xL
Dutta S, Gulla S, Chen TS, Fire E, Grant RA, Keating AE
J Mol Biol, 2010
The MCL-1 BH3 helix is an exclusive MCL-1 inhibitor and apoptosis sensitizer
Stewart ML, Fire E, Keating AE, Walensky LD
Nat Chem Biol, 2010
Identifying and reducing error in cluster-expansion approximations of protein energies
Hahn S, Ashenberg O, Grigoryan G, Keating AE
J Comput Chem, 2010
Design of protein-interaction specificity gives selective bZIP-binding peptides
Grigoryan G, Reinke AW, Keating AE
Nature, 2009
A computationally guided protein-interaction screen uncovers coiled-coil interactions involved in vesicular trafficking
Zhang H, Chen J, Wang Y, Peng L, Dong X, Lu Y, Keating AE, Jiang T
J Mol Biol, 2009
X-ray vs. NMR structures as templates for computational protein design
Schneider M, Fu X, Keating AE
Proteins, 2009
Cluster expansion models for flexible-backbone protein energetics
Apgar JR, Hahn S, Grigoryan G, Keating AE
J Comput Chem, 2009
Structural specificity in coiled-coil interactions
Grigoryan G, Keating AE
Curr Opin Struct Biol, 2008
Predicting helix orientation for coiled-coil dimers
Apgar JR, Gutwin KN, Keating AE
Proteins, 2008
Analysis of coiled-coil interactions between core proteins of the spindle pole body
Zizlsperger N, Malashkevich VN, Pillay S, Keating AE
Biochemistry, 2008
Modeling backbone flexibility to achieve sequence diversity: the design of novel alpha-helical ligands for Bcl-xL
Fu X, Apgar JR, Keating AE
J Mol Biol, 2007
Computing van der Waals energies in the context of the rotamer approximation
Grigoryan G, Ochoa A, Keating AE
Proteins, 2007
Paircoil2: improved prediction of coiled coils from sequence
McDonnell AV, Jiang T, Keating AE, Berger B
Bioinformatics, 2006
Structure-based prediction of bZIP partnering specificity
Grigoryan G, Keating AE
J Mol Biol, 2006
Ultra-fast evaluation of protein energies directly from sequence
Grigoryan G, Zhou F, Lustig SR, Ceder G, Morgan D, Keating AE
PLoS Comput Biol, 2006
Design of a heterospecific, tetrameric, 21-residue miniprotein with mixed alpha/beta structure
Ali MH, Taylor CM, Grigoryan G, Allen KN, Imperiali B, Keating AE
Structure, 2005
AVID: an integrative framework for discovering functional relationships among proteins
Jiang T, Keating AE
BMC Bioinformatics, 2005
Coarse-graining protein energetics in sequence variables
Zhou F, Grigoryan G, Lustig SR, Keating AE, Ceder G, Morgan D
Phys Rev Lett, 2005
Orientation and oligomerization specificity of the Bcr coiled-coil oligomerization domain
Taylor CM, Keating AE
Biochemistry, 2005
Predicting specificity in bZIP coiled-coil protein interactions
Fong JH, Keating AE, Singh M
Genome Biol, 2004
Comprehensive identification of human bZIP interactions with coiled-coil arrays
Newman JR, Keating AE
Science, 2003